Current Protocols Videos
- Calibration of the needle with masses
- Chromosomal stretching experiment using a Xenopus in vitro-assembled mitotic chromosome (Introduction/Basic Protocol)
- Coating grids with Formvar
- Counting and Determining the Viability of Cultured Cells
- Fixation of cells for cryo-immunogold labeling
- Freezing, Thawing, and Packaging Cells for Transport
- Labeling and washing grid-mounted sections
- Methylcellulose embedding
- Picking up sections after ultrathin sectioning
- Plunge freezing
- Preparing a mounted eyelash for handling sections
- Sucrose infiltration tissues/cells fixed for cryo-immunogold labeling
- Trypsinizing and Subculturing Mammalian Cells
- Tubule formation on a vesicle during endocytosis
- Ultrathin cryo-sectioning
- Ultrathin section retrieval and transfer to Formvar-coated grids
- Preparation and fractionation of xenopus laevis egg extracts
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